Potential of Lactic Acid Bacteria Isolated From Dangke and Indonesian Beef as Hypocholesterolaemic Agent

Lactobacillus fermentum strains were successfully isolated from dangke which was a fresh cheese-like product originating from Enrekang, South Sulawesi Province, Indonesia. In addition, Lactobacillus plantarum and Lactobacillus acidophillus were isolated from beef. This study aimed to investigate the ability of those 8 LAB strains from dangke and beef in lowering cholesterol level by using in vitro study. Strain of Lactic acid bacteria used were L. fermentum strains (A323L, B111K, B323K, C113L, C212L), L. plantarum strains (IIA-1A5 and IIA-2C12), and L. acidophillus IIA-2B4. Variables observed were identification of Bile Salt Hydrolase (BSH) gene by Polymerase Chain Reaction (PCR), BSH activity and cholesterol assimilation. Phylogenetic tree indicated homology of L. plantarum IIA-IA5 was 98% to BSH gene of L. plantarum Lp529 with access code of FJ439771 and FJ439775 obtained from GenBank. The results demonstrated that eight strains of LAB isolated from dangke and beef that potentially showed cholesterol-lowering effects were L. fermentum B111K and L. plantarum IIA-1A5. L. fermentum B111K was able to assimilate cholesterol by 4.10% with assimilated cholesterol of 0.13 mg in 1010 cells. In addition, L. plantarum IIA-1A5 had BSH gene and BSH activity, as well as the ability to assimilate cholesterol by 8.10% with assimilated cholesterol of 0.06 mg in 1010 cells. It is concluded that L. fermentum B111K and L. plantarum IIA-1A5 were strains that showed cholesterol-lowering effects

KESULTANAN BUTON PADA MASA PEMERINTAHAN SULTAN ANHARUDDIN: 1823-1824

Tujuan penelitian ini adalah untuk mendeskripsikan profil  Sultan Anharuddin yang menjadi Sultan Buton ke-28 pada tahun 1823-1824, untuk mengetahui proses pengangkatan Anharuddin sebagai Sultan Buton pada tahun 1823, serta untuk  mengetahui kondisi Kesultanan Buton pada masa pemerintahan Sultan Anharuddin. Metode yang digunakan dalam penelitian ini adalah metode sejarah yang terdiri dari lima tahap kegiatan, yaitu: (a) Pemilihan topik; (b) Heuristik sumber; (c) Verifikasi sumber; (d) Interpretasi, dan (e) Historiografi. Hasil penelitian menunjukkan bahwa Sultan Anharuddin atau La Dani, yang lahir pada tanggal 4 Rajab 1152  Hijriah,  merupakan keturunan  bangsawan dari golongan Tapi-Tapi. Orang tua Sultan Anharuddin bernama La Masalimu  dan ibunya  bernama Wa Ode Bawine Putri Kapitalao Itembana Walanda La Ode Ungkuabuso. Sultan Anharuddin menikah dengan Wa Ode Mufti. Anak Sultan Anharuddin dan Wa Ode Mufti adalah Wa Ode Baawu, Wa Ode Moha Raja Tiworo, Wa Ode Ramli Wolowo, Wa Ode Hinusu, Sapati Baadia, dan Wa Ode Aidi Baluna Kapitalao Waale-ale. Proses pengangkatan Anharuddin sebagai Sultan Buton dilakukan setelah  Sultan Anharuddin berhasil mengusir Bajak Laut Tobelo. Pemerintahan Sultan Anharuddin sebagai seorang sultan tidak berjalan dengan baik. Hal ini terjadi karena Sultan Anharuddin tidak bersedia menjalankan isi kontrak Dewan Syara Kesultanan Buton dengan pemerintah Belanda sehingga keadaan ekonomi, sosial, dan budaya pada masa pemerintahannya tidak berjalan dengan baik. Kondisi pertahanan dan keamanan Kesultanan Buton pada masa pemerintahan Sultan Anharuddin juga kurang kondusif

Time-Cost-Quality Trade-off Model for Optimal Pile Type Selection Using Discrete Particle Swarm Optimization Algorithm

The cost of pile foundations is part of the super structure cost, and it became necessary to reduce this cost by studying the pile types then decision-making in the selection of the optimal pile type in terms of cost and time of production and quality .So The main objective of this study is to solve the time–cost–quality trade-off (TCQT) problem by finding an optimal pile type with the target of "minimizing" cost and time while "maximizing" quality. There are many types In the world of piles but  in this paper, the researcher proposed five pile types, one of them is not a traditional, and   developed a model for the problem and then employed particle swarm optimization (PSO) algorithm, as one of evolutionary algorithms with the help of (Mat lab software), as a tool for decision making problem about choosing the best alternative of the traded piles, and proposes a multi objective optimization model, which aims to optimize the time, cost and quality of the pile types, and assist in selecting the most appropriate pile types. The researcher selected 10 of senior engineers to conduct interviews with them.  And prepared some questions for interviews and open questionnaire. The individuals are selected from private and state sectors each one have 10 years or more experience in pile foundations work. From personal interviews and field survey the research has shown that most of the experts, engineers are not fully aware of new soft wear techniques to helps them in choosing alternatives, despite their belief in the usefulness of using modern technology and software. The Problem is multi objective optimization problem, so after running the PSO algorithm it is usual to have more than one optimal solution, for five proposed pile types, finally the researcher  evaluated and  discussed the output results and  found out that pre-high tension spun (PHC)pile type was the optimal pile type

Pengaruh Sosialisasi Perpajakan, Pengetahuan Perpajakan, Persepsi Wajib Pajak Tentang Sanksi Pajak dan Implementasi Pp Nomor 46 Tahun 2013 terhadap Kepatuhan Wajib Pajak Orang Pribadi ( Studi Empiris pada Wajib Pajak di Kabupaten Banjarnegara )

This study aimed to analyze the factors that affect an individual taxpayer compliance in Banjarnegara. This study consists of four independent variables and the dependent variable. independent variable in this study is the socialization of taxation, tax knowledge, perceptions taxpayer about tax penalties and taxpayers perception on the implementation of PP 46 tahun2013. While the dependent variable in this study is the individual taxpayer compliance.This study used a simple random sampling technique and using the questionnaire survey method in data collection, respondents were sampled individual taxpayers who are enrolled in KP2KP Banjarnegara. Research data analysis using multiple regression analysis with SPSS 16.0.Based on the analysis that has been done, this study shows that the socialization of taxation, tax knowledge, and perceptions of the taxpayer of PP 46 in 2013 and a significant positive effect on the individual taxpayer compliance. While the taxpayer perceptions about tax penalties do not affect the compliance of individual taxpayers

Pengaruh Penambahan Polypropylene Fiber Mesh Terhadap Sifat Mekanis Beton

Polypropylene fiber is the fiber type of plastic (polypropylene) which is a special production with high technology. It is a chemical compound with the formula C3H6 hydrocarbons in the form of a single filamentor thin fibrous tissue shaped nets with a length of 6 mm to 50 mm and has a diameter of 8-90 microns. Thesefibers can be used to improve the performance of concrete.This research aims to determine how much influence the addition of polypropylene fibers on mechanicalproperties of concrete (compressive strength. split tensile strength and flexural strength). polypropylenefibers used doses of 0; 0.40 kg/m³, 0.60 kg/m³, 0.80 kg/m³ of concrete, which is made on the type of concretef'c 20 MPa with the value of water cement factor (FAS) at 0.53. Calculation of mixed concrete plans usingguidelines DOE (Department of Environment). The research location at Concrete and Building MaterialsLaboratory of the Faculty of Engineering, University Tadulako Palu. Specimens made as many as 72 piecesof 60 cylinders (D15 x 30 cm ³) and a beam of 12 pieces (15 x 15 x 60 cm ³). Specimen testing performed at28 days.Test results obtained fiber concrete compressive strength, split tensile strength and flexural strength, higherthan normal concrete. Compressive strength testing of optimum values obtained at doses 0.60 kg/m³. with anincrease of 3.62% compared to normal concrete. From testing split tensile strength and flexural strengthoptimum values obtained of each at doses of 0.65 kg/m³ and 0.58 kg/m³. For the split tensile strengthincreased 20.44% and flexural strength increased 11.26% compared to normal concrete

Where energy flows, passion grows: testing a moderated mediation model of work passion through a cross-cultural lens

This study examines how and when passionate leaders can instigate work passion in their followers. We propose relational energy as a social interaction mediator that can facilitate the crossover of work passion from leader to followers. Additionally, we introduce a moderator of culture (Anglo culture, e.g., Canada vs. Confucian Asian culture, e.g., China) as it plays a vital role in the dynamics of interpersonal relations within a leader-follower dyad. We collected two-wave data from MBA students of two Confucian Asian countries (China and Singapore, n = 120) and two Anglo countries (Canada and Australia, n = 265) to test our moderated mediation model. The results show that interactions with passionate leaders can generate relational energy in followers and subsequently lead to followers’ passion for work. Furthermore, the findings shed light on the moderating effect of culture, such that the leader-follower work passion relationship via follower relational energy was stronger for followers from Anglo culture than the followers from Confucian Asian culture. Limitations of the study and directions for future research are discussed

Terminal differentiation is not a major determinant for the success of stem cell therapy - cross-talk between muscle-derived stem cells and host cells

We have found that when muscle-derived stem cells (MDSCs) are implanted into a variety of tissues only a small fraction of the donor cells can be found within the regenerated tissues and the vast majority of cells are host derived. This observation has also been documented by other investigators using a variety of different stem cell types. It is speculated that the transplanted stem cells release factors that modulate repair indirectly by mobilizing the host's cells and attracting them to the injury site in a paracrine manner. This process is loosely called a 'paracrine mechanism', but its effects are not necessarily restricted to the injury site. In support of this speculation, it has been reported that increasing angiogenesis leads to an improvement of cardiac function, while inhibiting angiogenesis reduces the regeneration capacity of the stem cells in the injured vascularized tissues. This observation supports the finding that most of the cells that contribute to the repair process are indeed chemo-attracted to the injury site, potentially through host neo-angiogenesis. Since it has recently been observed that cells residing within the walls of blood vessels (endothelial cells and pericytes) appear to represent an origin for post-natal stem cells, it is tempting to hypothesize that the promotion of tissue repair, via neo-angiogenesis, involves these blood vessel-derived stem cells. For non-vascularized tissues, such as articular cartilage, the regenerative property of the injected stem cells still promotes a paracrine, or bystander, effect, which involves the resident cells found within the injured microenvironment, albeit not through the promotion of angiogenesis. In this paper, we review the current knowledge of post-natal stem cell therapy and demonstrate the influence that implanted stem cells have on the tissue regeneration and repair process. We argue that the terminal differentiation capacity of implanted stem cells is not the major determinant of the cells regenerative potential and that the paracrine effect imparted by the transplanted cells plays a greater role in the regeneration process

Desain Sistem Pipa Distribusi Gas untuk Sektor Perumahan dan Komersial di Wilayah Kebayoran Baru – Jakarta Selatan

Pemanfaatan gas alam di Indonesia untuk sektor Perumahan dan komersial masih belum optimum, termasuk di wilayah ibukota. Berbagai kebijakan telah dirumuskan pemerintah untuk mendukung pemanfaatan gas alam termasuk meningkatkan infrastruktur penyaluran gas alam. Tujuan utama dari studi ini adalah untuk melakukan perancangan sistem pipa distribusi gas untuk sektor Perumahan dan komersial di wilayah Kebayoran Baru (Jakarta Selatan) serta melakukan analisa kelayakan ekonomi dari proyek tersebut. Kebayoran Baru adalah wilayah yang potensial untuk dilakukan pengembangan gas kota. Berdasarkan perhitungan yang telah dilakukan di studi ini, kebutuhan gas untuk sektor Perumahan dan komersial di Kebayoran Baru adalah sekitar 0,9 MMSCFD. Studi ini mencakup pula pemilihan rute untuk sistem pipa distribusi gas di wilayah tersebut. Berdasarkan hasil simulasi dengan perangkat lunak Fluid Flow dan dikonversi dengan spesifikasi pipa komersial yang ada di pasaran, diameter pipa yang akan digunakan adalah 180 mm, 90 mm, dan 63 mm. Total panjang pipa distribusi tersebut adalah 20,3 m. Dari hasil studi ini, rancangan tersebut akan layak dari segi ekonomi apabila harga jual gas sebesar Rp3.141/m3dan harga ini lebih rendah dibandingkan harga LPG untuk wilayah Jakarta